Estrogenic Performance of High Doses of Lignans in Immature Female Mice

Aous Kahtan Almzaien

Abstract

This study was conducted to evaluate the responses of immature female mice to high doses of Lignans intubated orally for four weeks period. Diethyl ether and 80% ethanol was used for extraction of Lignans from flaxseeds. Thirty immature female mice, 21 one day old, were divided randomly into three equal groups were administered 50 and 100 mg/kg body of Lignans, respectively while the third group served as a control. The results showed that no significant differences in body weight gain among Lignans treated groups as compared to the control was recorded. On the other hand, Lignans had induced a significant increment in uterine and ovarian weight compared with control groups. However, reproductive organs, weight increments were coincided with the significant increments of gonadotropic hormones; follicle-stimulating hormone (FSH), Luteinizing hormone (LH) and estrogen levels. Moreover, thyroid hormones showed a significant alteration manifested by a significant decrease in the levels of triiodothyronine (T3) and thyroxine (T4) and a significant increase in thyroid stimulating hormone (TSH). Interestingly, these results confirmed the estrogenic activity of high doses of Lignans extracted from flaxseed.