Elevation of IL-32 and TNF-α and 23S rRNA Gene Mutations in Helicobacter pylori Infected Iraqi Patients

Safa S. Abd

Abstract

Helicobacter pylori infection is a common bacterial infection of the stomach that often causes no symptoms, but can lead to gastritis, peptic ulcers, and in some cases, stomach cancer. In this study, (50) blood specimens were taken from patients who had H. pylori infection, and from (40) healthy individuals as a control groups in Baghdad teaching Hospital during the period from January to October 2025. The results of the current study showed that the mean level of H. pylori IgM antibodies was significantly higher in patients (2.14±0.17) compared to controls (0.10±0.03), and similarly, the mean IgG level was significantly higher in patients (14.31±0.94) compared to controls (0.11±0.03) (P≤0.01). Moreover, the mean level of IL-32 among the H. pylori infected patients was (27.06±2.23) compared to the healthy persons (1.25±0.32), also, the mean level of TNF-α among patients was (18.06±0.79) compared to the healthy persons (1.76±0.39), with highly significant differences (P≤0.01) respectively. In the 23S rRNA gene, the A2143G locus mutated from GG to GA and AA, while the A2142G locus mutated from AA to AG and GG.