pdf The Role of SnoRNA-21, FOXD2-AS1on Regulated the Gene Expression of Mis-Match Repair Gene in Male with Lung Cancer

Mohammed S. Khudher and Owayes M. Hamed

Abstract

Lung cancer is a common cancer worldwide and can occur due to various causes, one of which is the MMR mismatch repair system. This system repairs mismatched nucleotides in the DNA strand during replication. MLH1, a key gene in the MMR system, is essential in repairing mutations in lung cancer patients. Certain non-coding RNA genes, such as snoRNA and FOXD2-AS1, can regulate the expression of this gene. These non-coding RNAs influence MLH1 gene expression in lung cancer patients. The examination targeted at detecting the impact of miRNA and non-coding RNA on MLH1 gene expression rates in lung cancer patients situated within Dohuk City. The study included 70 patients aged between 45 and 65. The sample was divided into two groups: one included 55 lung cancer patients; another group included 15 patient controls. The study consisted of two stages: first, detecting the level of MLH1 gene expression; second, assessing the level of non-coding RNA influencing gene activity. The results showed that the MLH1 gene expression value was 0.38, while the gene expression values for non-coding RNAs were 4.4 for snoRNA and 4.2 for FOXD2-AS1.