pdf Sequencing and Phylogenetic Tree in Cat and Tet B Gene in Patients with Salmonella Typhi

Dr. Inas Sattar Abd, Hiba Nabil Abbas

Abstract

Salmonella typhi is resistant to numerous antibiotics because recurrently treated per antibiotic.  on bases the positive and strong isolates, we selected six isolates for Tet B gene and three isolates for Cat for sequencing analysis .  This study detected genetic sequencing and phylogenetic relationships of Tet B and cat genes from patients infected with Salmonella typhi. during specific gene analysis , which detected present two mutations (silent) were identified in gene ( cat) that that are protein remain same , while 14 mutation occur in Tet B gene were silent and another were missenses mutation. Phylogenetic analysis revealed that Salmonella typhi was similar to other salmonella on cat gene and tet B gene.

          Aim of the study to appreciate Slamonella typhi role in antibiotic resistance mechanisms through detected mutation that occur in cat and tet B gene and their evolution within numerous strains of Salmonella typhi..