Histopathological Study for Biofilm-Forming Klebsiella pneumoniae

Abstract

The goal of this investigation was to ascertain if biofilm-forming Klebsiella pneumoniae isolates may cause histopathological alterations. Thirty-eight K. pneumoniae isolates were obtained from patients who visited two hospitals in Baghdad; each isolate was identified using biochemical, microscopic, and cultural characteristics. Polystyrene microtiter plates were used to assess the K. pneumoniae isolates' capacity to produce biofilm. The degree of biofilm thickness was reflected in the optical density (OD), which was measured at 630 nm. Approximately 21.05% of the tested isolates were weakly adhering, 73.6% were moderately adherent, and 5.26% were strongly adherent, according to the results. Isolate K22 was comparatively biofilm-forming, while isolate K20 was poor. Eighteen male Swiss white mice (Mus musculus) were used to test these two isolates intranasally. Weak biofilm former K. pneumoniae K20 caused less lung damage than moderate biofilm former K. pneumoniae K22, with pathology scores of 9 and 13, respectively, according to histopathological abnormalities in mice's lungs.