The Study of lytA Genotyping and estimation of IL17A and TGF-β in Streptococcus pneumoniae patients
Abstract
Streptococcus pneumoniae is regarded as a main cause of CAP among other pathogens. In the current study, (60) patients infected with Streptococcus pneumoniae were included, from whom blood samples were collected. The patients attended to Baghdad Teaching Hospital during the period from 1st October 2023 to 20th April 2024. Blood sample also obtained from (60) apparently healthy individuals as controls. The results showed that the prevalence of streptococcus pneumonia infection was higher among the age groups (25-34) and (45-54) as compared to other age groups. Also, the distribution of streptococcus pneumonia infection was higher among males 31(62.0%) than females 19(38.0%) with no significant variation. The prevalence of streptococcus pneumonia infection according to residency was equal between rural and urban patients. A highly significant variation (P<0.01) was found between the mean Strept. IgM (2.1164±1.27) in the patient group and the healthy controls (0.05±0.04), and between the (Mean±Sd) of Strept. IgG (1.72±0.75) in the patients and the healthy controls (0.04±0.02). Also, the (Mean±Sd) IL17A was significantly higher (p<0.01) in the patient group (96.41±26.11) than the control group (19.68±6.52), and the (Mean±Sd) TGF was significantly higher (p<0.01) higher in the patient group (101.74±32.88) than the control group (23.59±10.86). There was a highly significant variation (p<0.01) between mean IL-17A and Anti Strept. IgM antibodies (25.423). There was a highly significant variation (p<0.01) between mean value IL-17A and Anti Streptococcus pneumoniae IgG antibodies (25.684), and a highly significant variation (p<0.01) between mean value TGF and Anti Strept. IgM antibodies (2.104). Also there was a highly significant variation (p<0.01) between mean value TGF and Anti Strept IgG antibodies (21.298), and a highly significant difference (p<0.01) between mean value of TGF and IL-17A (21.592). Results of amplification of specific region of lytA gene of Streptococcus pneumonia. The 1.5% agarose gel electrophoresis was used for fractionation of species of human samples and stained by ethidium bromide M: 100bp ladder markers. Lane 1-15 and 1-15 resembled 1023bp PCR product.
